General Information
Name: Di Dong-Wei
Title: Associate Professor
Work Email: dwdi@issas.ac.cn
Work Phone: 025-86881552
Research Fields
(1)Molecular response mechanism of ammonium (NH4+) toxicity in Arabidopsis and rice
Education Background
(1)2010 – 2015, Ph.D., Cell Biology, Lanzhou University, Lanzhou, China
(2)2006 – 2010, Bachelor, Biotechnology, Hefei University of Technology, Hefei, China
Professional Experience
(1)2022 ― Present, Associate Professor, Institute of Soil Science, CAS, Nanjing, China
(2)2019 ― 2022, Research Assistant, Institute of Soil Science, CAS, Nanjing, China
(3)2015 ― 2019, Postdoctoral Fellow, Institute of Soil Science, CAS, Nanjing, China
Projects
(1)Biochemical and molecular mechanisms of OsAMOS1 in rice adapting to high ammonium, 2017.01-2019.12, National Natural Science Foundation of China (PI)
(2)Study on the molecular mechanism of OsAMOS1 protein in rice adapting to high ammonium, 2017.05-2019.05, China Postdoctoral Science Foundation (PI)
(3)Study on the function of OsAMOS1 in rice adapting to high ammonium, 2015.08-2017.07, China Postdoctoral Science Foundation (PI)
Publications
First author (#) or corresponding author (*):
(1) Luo, P.#,*, Di, D. W.#,*, Wu, L., Yang, J., Lu, Y. and Shi, W. MicroRNAs are Involved in Regulating Plant Development and Stress Response through Fine-Tuning of TIR1/AFB-dependent Auxin Signaling. International Journal of Molecular Sciences, 2022, 23(1), 510. (Co-first/corresponding author)
(2) Di, D.W. #, Sun, L., Wang, M., Wu, J., Kronzucker, H.J., Fang, S., Chu, J. Shi, W. & Li, G*. WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and IAA-conjugating genes and by inhibiting NH4+ efflux in the root elongation zone. New Phytologist, 2021, 232: 190-217.
(3) Di, D.W. #, Li, G., Sun, L., Wu, J., Wang, M., Kronzucker, H.J., Fang, S., Chu, J. & Shi, W*. High ammonium inhibits root growth in Arabidopsis thaliana by promoting auxin conjugation rather than inhibiting auxin biosynthesis. Journal of Plant Physiology, 2021, 261: 153415.
(4) Sun, L.#, Di, D. W.#, Li, G., Li, Y., Kronzucker, H. J., & Shi, W*. Transcriptome analysis of rice (Oryza sativa L.) in response to ammonium resupply reveals the involvement of phytohormone signaling and the transcription factor OsJAZ9 in reprogramming of nitrogen uptake and metabolism. Journal of Plant Physiology, 2020, 246-247: 153137. (Co-first author)
(5) Sun, L.#, Di, D. W.#, Li, G., Li, Y., Kronzucker, H. J., Wu, X., & Shi, W*. Endogenous ABA alleviates rice ammonium toxicity by reducing ROS and free ammonium via regulation of the SAPK9-bZIP20 pathway. Journal of Experimental Botany, 2020, 71(15): 4562-4577. (Co-first author)
(6) Di, D.W. #, Sun, L., Zhang, X., Li, G., Kronzucker, H. J., & Shi, W*. Involvement of auxin in the regulation of ammonium tolerance in rice (Oryza sativa L.). Plant and Soil, 2018, 432: 373-387.
(7) Di, D. W. #, Wu, L., Zhang, L., An, C. W., Zhang, T. Z., Luo, P., ... & Guo, G. Q*. Functional roles of Arabidopsis CKRC2/YUCCA8 gene and the involvement of PIF4 in the regulation of auxin biosynthesis by cytokinin. Scientific Reports, 2016, 6, 36866.
(8) Di, D. W. #,*, Wu, L., Luo, P., Zhang, L., Zhang, T. Z., Sun, X., ... & Guo, G. Q. Analysis the role of Arabidopsis CKRC6/ASA1 in auxin and cytokinin biosynthesis. Journal of Plant Biology, 2016, 59(2): 162-171.
(9) Di, D. W. #,*, Zhang, C., Luo, P., An, C. W., & Guo, G. Q. The biosynthesis of auxin: how many paths truly lead to IAA?. Plant Growth Regulation, 2016, 78(3): 275-285.
(10) Di, D. W. #,*, Zhang, C., & Guo, G. Q. Involvement of secondary messengers and small organic molecules in auxin perception and signaling. Plant Cell Reports. 2015, 34(6): 895-904.
(11) Wu, L.#, Luo, P.#, Di, D. W.#, Wang, L., Wang, M., Lu, C. K., Zhang, L., Zhang, T. Z., NOVáK, O. & Guo, G. Q*. Forward genetic screen for auxin-deficient mutants via cytokinin-induced root curling. Scientific Reports, 2015, 5(1): 11923. (Co-first author)
(12) Wu, L.#, Di, D. W.#, Zhang, Dan., Song B. & Guo, G. Q*. Frequent problems and their resolutions by using thermal asymmetric interlaced PCR (TAIL-PCR) to clone genes in Arabidopsis T-DNA tagged mutants. Biotechnology & Biotechnological Equipment. 2015, 29(2):260-267. (Co-first author)
Co-author:
(1) Wang J., Di D-W., Luo P., Zhang L., Li X-F., Guo G-Q and Wu L. The roles of epigenetic modifications in the regulation of auxin biosynthesis. Frontiers in Plant Science, 2022, 959053.
(2) Li G., Zhang L., Wu J., Yue X., Wang M., Sun L., Di D., Kronzucker J.H.*, Shi W.* OsEIL1 protects rice growth under NH4+ nutrition by regulating OsVTC1-3-dependent N-glycosylation and root NH4+ efflux. Plant Cell and Environment, 2022, https://doi.org/10.1111/pce.14283.
(3) Wu, J., Lu, Y., Di, D., Cai, Y., Zhang, C., Kronzucker, H.J., Shi, W*. and Gu, K*. OsGF14b is involved in regulating coarse root and fine root biomass partitioning in response to elevated [CO2] in rice. Journal of Plant Physiology, 2021, https://doi.org/10.1016/j.jplph.2021.153586.
(4) Zhang, L.#, Song, H.#, Li, B., Wang, M., Di, D., Lin, X., Kronzucker, HJ., Shi, W., Li., G*. Induction of S-nitrosoglutathione reductase protects root growth from ammonium toxicity by regulating potassium homeostasis in Arabidopsis and rice. Journal of Experimental Botany. 2021, erab140.
(5) Zhang, L., Luo, P., Bai, J., Wu, L., Di, D. W., & Liu, H. Q., et al. Function of histone H2B monoubiquitination in transcriptional regulation of auxin biosynthesis in Arabidopsis. Communications Biology, 2021, 4: 206.
(6) Chen, M., Chen, G., Di, D., Kronzucker, H. J., & Shi, W*. Higher nitrogen use efficiency (NUE) in hybrid "super rice" links to improved morphological and physiological traits in seedling roots. Journal of Plant Physiology, 2020, 251: 153191.
(7) Wang, M., Zhang, P., Liu, Q., Li, G., Di, D., Xia, G.*, Kronzucker, H. J., Fang, S., Chu, J. & Shi, W*. TaANR1-TaBG1 and TaWabi5-TaNRT2s/NARs link ABA metabolism and nitrate acquisition in wheat roots. Plant Physiology, 2020, 182 (3): 1440-1453.
(8) Zhang, J., Hafeez, M. T., Di, D., Wu, L., & Zhang, L*. Precise control of aba signaling through post-translational protein modification. Plant Growth Regulation, 2019, 88: 99-111.
(9) Li, G., Zhang, L., Wang, M., Di, D., Kronzucker, H. J., & Shi, W*. The Arabidopsis AMOT1/EIN3 gene plays an important role in the amelioration of ammonium toxicity. Journal of Experimental Botany, 2019, 70(4): 1375-1388.
(10) Zhang, L., Li, G., Dong, G., Wang, M., Di, D., Kronzucker, H. J., & Shi, W*. Characterization and comparison of nitrate fluxes in tamarix ramosissima and cotton roots under simulated drought conditions. Tree Physiology, 2018, 39 (4): 628-640.
(11) Zhang, L., Li, G., Wang, M., Di, D., Sun, L., Kronzucker, H. J., & Shi, W*. Excess iron stress reduces root tip zone growth through nitric oxide-mediated repression of potassium homeostasis in Arabidopsis. New Phytologist, 2018, 1: 259-274.
(12) Li, S., Di, D., Li, G., Kronzucker, H. J., & Shi, W*. Spatio-temporal dynamics in global rice gene expression (Oryza sativa L.) in response to high ammonium stress. Journal of Plant Physiology, 2017, 212: 94-104.
